Annals of Coloproctology

Review

Gene and protein expression of epithelial to mesenchymal transition for intestinal and anal fistula: a systematic review: Nadila Haryani Osman, Ruhi Fadzlyana Jailani, Hayati Abd Rahman, Nazefah Abdul Hamid; Ann Coloproctol. 2023;39(2):106-114. Published online December 3, 2021; DOI: https://doi.org/10.3393/ac.2021.00584.0083

3,428 View
170 Download
2 Web of Science
2 Citations

Purpose
Intestinal fibrosis is a common complication of inflammatory bowel diseases. However, the possible involvement of epithelial-mesenchymal transition (EMT) has been scarcely investigated. This systematic review aims to search through research papers that are focusing on messenger RNA (mRNA) and protein expression profile in EMT in fistula or in intestinal fibrosis.
Methods
Electronic exploration was performed until April 24, 2019 through PubMed, Ovid, Science Direct, and Scopus databases with the terms of “fistula” OR “intestinal fibrosis” AND “epithelial-mesenchymal transition”. Two independent reviewers scrutinized the suitability of the title and abstract before examining the full text that met the inclusion criteria. For each study, the sample types that were used, methods for analysis, and genes expressed were identified. The list of genes was further analyzed using DAVID (Database for Annotation, Visualization, and Integrated Discovery) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway.
Results
There were 896 citations found; however, only 3 studies fulfilled the requirements. Among the EMT-related genes, 5 were upregulated genes at mRNA level while 6 were at protein level. However, only 2 downregulated genes were found at each mRNA and protein level. Of the 4 inflammation-related genes found, 3 genes were upregulated at mRNA level and 1 at protein level. These genes were confirmed to be involved in the development of inflammatory induced fibrosis and fistula through EMT. Results from quantitative real-time polymerase chain reaction analysis were consistent with the process of EMT, confirmed by the western blot protein analysis.
Conclusion
Many significant genes which are involved in the process of EMT in fistula and intestinal fibrosis have been identified. With high-end technology many more genes could be identified. These genes will be good molecular targets in the development of biomarkers for precision drug targeting in the future treatment of intestinal fibrosis and fistula.

Citations

Citations to this article as recorded by

Role of Adipose Tissue Hormones in Pathogenesis of Cryptoglandular Anal Fistula
Marcin Włodarczyk, Jakub Włodarczyk, Kasper Maryńczak, Anna Waśniewska-Włodarczyk, Urszula Doboszewska, Piotr Wlaź, Łukasz Dziki, Jakub Fichna
International Journal of Molecular Sciences.2024; 25(3): 1501. CrossRef
Exosomes Derived from Colon Cancer Cells Promote Tumor Progression and Affect the Tumor Microenvironment
Minsung Kim, Il Tae Son, Gyoung Tae Noh, So-Youn Woo, Ryung-Ah Lee, Bo Young Oh
Journal of Clinical Medicine.2023; 12(12): 3905. CrossRef

Original Article

Gland Attenuation, a Novel Morphological Feature of Colorectal Cancer: Evidence for an Epithelial-Mesenchymal Transition: Tae-Hwa Baek, Dong-Wook Kang, Joo-Heon Kim, Hyun-Jin Son; Ann Coloproctol. 2018;34(4):187-196. Published online August 31, 2018; DOI: https://doi.org/10.3393/ac.2017.12.02

4,306 View
54 Download
4 Web of Science
4 Citations

Abstract PDF

Purpose
Along the invasive margin, colorectal cancer may show distinctive morphologic changes characterized by an asymmetrically attenuating tumor gland with loss of polarity. The author coined the term ‘gland attenuation (GA)’ for these peculiar changes. The aims of this study were to compare the immunoreactivity of the epithelial-mesenchymal transition (EMT) markers E-cadherin and β-catenin and thus determine whether EMTs occurs at tumor budding (TB) or GA sites and to assess the association of TB and/or GA levels with clinicopathological parameters and prognosis.
Methods
Expression patterns of E-cadherin and β-catenin in the tumor centers at GA and TB sites were examined in 101 patients with well or moderately differentiated CRCs, and the prognostic significance of TB and/or GA was statistically evaluated.
Results
GA foci, as well as TB foci, revealed loss of membranous and cytoplasmic E-cadherin expressions and aberrant β-catenin expression with reduced membranous expression and increased localization to the nucleus, suggesting that EMTs occur in GA as well as in TB. The high-TB and the TB-dominant groups were significantly correlated with advanced invasion depth, presence of lymph node metastasis, advanced pathologic staging and presence of lymphovascular invasion. The high-TB and the TB-dominant groups showed poor overall survival (OS) and recurrence-free survival (RFS), and high TB was an independent prognostic factor in the multivariate analyses for OS and RFS.
Conclusion
This study showed evidence that EMTs occurs at GA sites as well as TB foci. TB is a strong and independent prognostic factor, and TB-dominance may be an indicator of adverse clinical outcome.

Citations

Citations to this article as recorded by

Tumor budding as a potential prognostic marker in determining the behavior of primary liver cancers
Betul Unal, Mennan Yigitcan Celik, Elif Ocak Gedik, Cumhur Ibrahim Bassorgun, Gulsum Ozlem Elpek
World Journal of Hepatology.2023; 15(6): 775. CrossRef
Identification of a Novel Epithelial–Mesenchymal Transition Gene Signature Predicting Survival in Patients With HNSCC
Wei Xin, Chaoran Zhao, Longyang Jiang, Dongmei Pei, Lin Zhao, Chengpu Zhang
Pathology and Oncology Research.2021;[Epub] CrossRef
Prognostic impact of microscopic vessel invasion and visceral pleural invasion and their correlations with epithelial–mesenchymal transition, cancer stemness, and treatment failure in lung adenocarcinoma
Shinya Neri, Toshi Menju, Terumasa Sowa, Yojiro Yutaka, Daisuke Nakajima, Masatsugu Hamaji, Akihiro Ohsumi, Toyofumi F. Chen-Yoshikawa, Toshihiko Sato, Makoto Sonobe, Akihiko Yoshizawa, Hironori Haga, Hiroshi Date
Lung Cancer.2019; 128: 13. CrossRef
MicroRNA‐198‐5p inhibits the migration and invasion of non‐small lung cancer cells by targeting fucosyltransferase 8
Siyao Wang, Xin Zhang, Chunlu Yang, Shun Xu
Clinical and Experimental Pharmacology and Physiology.2019; 46(10): 955. CrossRef

First
Prev
Page of 1
Next
Last

Search