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1 "Nadila Haryani Osman"
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Gene and protein expression of epithelial to mesenchymal transition for intestinal and anal fistula: a systematic review
Nadila Haryani Osman, Ruhi Fadzlyana Jailani, Hayati Abd Rahman, Nazefah Abdul Hamid
Ann Coloproctol. 2023;39(2):106-114.   Published online December 3, 2021
DOI: https://doi.org/10.3393/ac.2021.00584.0083
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  • 2 Web of Science
  • 2 Citations
AbstractAbstract PDF
Purpose
Intestinal fibrosis is a common complication of inflammatory bowel diseases. However, the possible involvement of epithelial-mesenchymal transition (EMT) has been scarcely investigated. This systematic review aims to search through research papers that are focusing on messenger RNA (mRNA) and protein expression profile in EMT in fistula or in intestinal fibrosis.
Methods
Electronic exploration was performed until April 24, 2019 through PubMed, Ovid, Science Direct, and Scopus databases with the terms of “fistula” OR “intestinal fibrosis” AND “epithelial-mesenchymal transition”. Two independent reviewers scrutinized the suitability of the title and abstract before examining the full text that met the inclusion criteria. For each study, the sample types that were used, methods for analysis, and genes expressed were identified. The list of genes was further analyzed using DAVID (Database for Annotation, Visualization, and Integrated Discovery) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway.
Results
There were 896 citations found; however, only 3 studies fulfilled the requirements. Among the EMT-related genes, 5 were upregulated genes at mRNA level while 6 were at protein level. However, only 2 downregulated genes were found at each mRNA and protein level. Of the 4 inflammation-related genes found, 3 genes were upregulated at mRNA level and 1 at protein level. These genes were confirmed to be involved in the development of inflammatory induced fibrosis and fistula through EMT. Results from quantitative real-time polymerase chain reaction analysis were consistent with the process of EMT, confirmed by the western blot protein analysis.
Conclusion
Many significant genes which are involved in the process of EMT in fistula and intestinal fibrosis have been identified. With high-end technology many more genes could be identified. These genes will be good molecular targets in the development of biomarkers for precision drug targeting in the future treatment of intestinal fibrosis and fistula.

Citations

Citations to this article as recorded by  
  • Role of Adipose Tissue Hormones in Pathogenesis of Cryptoglandular Anal Fistula
    Marcin Włodarczyk, Jakub Włodarczyk, Kasper Maryńczak, Anna Waśniewska-Włodarczyk, Urszula Doboszewska, Piotr Wlaź, Łukasz Dziki, Jakub Fichna
    International Journal of Molecular Sciences.2024; 25(3): 1501.     CrossRef
  • Exosomes Derived from Colon Cancer Cells Promote Tumor Progression and Affect the Tumor Microenvironment
    Minsung Kim, Il Tae Son, Gyoung Tae Noh, So-Youn Woo, Ryung-Ah Lee, Bo Young Oh
    Journal of Clinical Medicine.2023; 12(12): 3905.     CrossRef

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