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Kyu Yun Jang 2 Articles
Concurrent Large Cell Neuroendocrine Carcinoma and Adenocarcinoma of the Ascending Colon: A Case Report
Yo Na Kim, Ho Sung Park, Kyu Yun Jang, Woo Sung Moon, Dong Geun Lee, Ho Lee, Min Ro Lee, Kyung Ryoul Kim
J Korean Soc Coloproctol. 2011;27(3):157-161.   Published online June 30, 2011
DOI: https://doi.org/10.3393/jksc.2011.27.3.157
  • 3,836 View
  • 23 Download
  • 9 Citations
AbstractAbstract PDF

Large cell neuroendocrine carcinomas of the colon are rare and represent only a small percentage of all colonic endocrine tumors. Here, we report a case of a colonic large cell neuroendocrine carcinomas concurrent with a colonic adenocarcinoma. A 70-year-old man presented with acute abdominal pain. A spiral computed tomography scan of the abdomen revealed eccentric wall thickening on the ascending colon. An explorative laparotomy and a right hemicolectomy were performed. Grossly, two separated masses were observed in the proximal ascending colon. One was a 7.4 × 5.1 cm ulcerative fungating lesion, and the other was a 2.8 × 1.9 cm polypoid lesion. Microscopically, the ulcerative fungating lesion showed a well-differentiated neuroendocrine morphology with necrosis and increased mitosis. Most of the tumor cells had large, vesicular nuclei with eosinophilic nucleoli, variable amounts of eosinophilic cytoplasm, and immunoreactivity for chromogranin A and synaptophysin. The polypoid lesion was a well-differentiated adenocarcinoma that had invaded the submucosa. We diagnosed these lesions as a concurrent large cell neuroendocrine carcinoma and an adenocarcinoma of the ascending colon.

Citations

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  • Large cell neuroendocrine tumor of the gastrointestinal tract with concurrent tubular adenomas
    Inayat GILL, Christienne SHAMS, Elisa QUIROZ, Subhashree M. KRISHNAN, Susanna GAIKAZIAN
    Gazzetta Medica Italiana Archivio per le Scienze Mediche.2021;[Epub]     CrossRef
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    Ali Allouch, Mohamad K. Moussa, Ali Dirany, Zahraa Barek, Mohammad Makke, Nizar Bitar
    International Journal of Surgery Case Reports.2020; 76: 421.     CrossRef
  • A case of colorectal large cell neuroendocrine carcinoma accompanied by disseminated peritoneal leiomyomatosis
    Kunihiko Suga, Hiroomi Ogawa, Makoto Sohda, Chika Katayama, Naoya Ozawa, Katsuya Osone, Takuhisa Okada, Takuya Shiraishi, Ryuji Katoh, Akihiko Sano, Makoto Sakai, Takehiko Yokobori, Ken Shirabe, Hiroshi Saeki
    Surgical Case Reports.2020;[Epub]     CrossRef
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    Tomoaki Yoshida, Kenya Kamimura, Kazunori Hosaka, Koji Doumori, Hiromitsu Oka, Akito Sato, Yasuo Fukuhara, Shoji Watanabe, Tomomi Sato, Akira Yoshikawa, Takashi Tomidokoro, Shuji Terai
    World Journal of Clinical Cases.2019; 7(14): 1865.     CrossRef
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    Jiancong Hu, Xiaochuan Chen, Dezheng Lin, Zhaoliang Yu, Juan Li, Xuefeng Guo
    International Journal of Surgery Oncology.2019; 4(6): e79.     CrossRef
  • Large Cell Neuroendocrine Carcinoma of the Rectum with Long Recurrence-free Survival after Radical Surgery and Adjuvant Chemotherapy
    Kukiko SAKIHAMA, Kinuko NAGAYOSHI, Hayato FUJITA, Shuntaro NAGAI, Yoshinao ODA, Masafumi NAKAMURA
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    ACG Case Reports Journal.2014; 1(2): 96.     CrossRef
  • Simultaneous Neuroendocrine Carcinoma and Adenocarcinoma of the Colon
    Donggeum Shin, Youngsook Park, Soojung Gong, Seonghwan Kim, Yunju Jo, Sangbong Ahn, Joonkil Han, Eunkyung Kim
    Intestinal Research.2013; 11(2): 137.     CrossRef
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    Yoshifumi Nakayama, Takayuki Torigoe, Noritaka Minagawa, Toshihito Uehara, Koji Yamaguchi
    Case Reports in Gastroenterology.2013; 7(1): 117.     CrossRef
Effects of Delay in the Snap Freezing of Colorectal Cancer Tissues on the Quality of DNA and RNA
Sang Hyun Hong, Hyun Ah Baek, Kyu Yun Jang, Myoung Ja Chung, Woo Sung Moon, Myoung Jae Kang, Dong Geun Lee, Ho Sung Park
J Korean Soc Coloproctol. 2010;26(5):316-323.   Published online October 31, 2010
DOI: https://doi.org/10.3393/jksc.2010.26.5.316
  • 4,623 View
  • 73 Download
  • 38 Citations
AbstractAbstract PDF
Purpose

The success of basic molecular research using biospecimens strongly depends on the quality of the specimen. In this study, we evaluated the effects of delayed freezing time on the stability of DNA and RNA in fresh frozen tissue from patients with colorectal cancer.

Methods

Tissues were frozen at 10, 30, 60, and 90 minutes after extirpation of colorectal cancer in 20 cases. Absorbance ratio of 260 to 280 nm (A260/A280) and agarose gel electrophoresis were evaluated. In addition, the RNA integrity number (RIN) was assayed for the analysis of the RNA integrity.

Results

Regardless of delayed freezing time, all DNA and RNA samples revealed A260/A280 ratios of more than 1.9, and all DNA samples showed a discrete, high-molecular-weight band on agarose gel electrophoresis. The RINs were 7.53 ± 2.04, 6.70 ± 1.88, 6.47 ± 2.58, and 4.22 ± 2.34 at 10, 30, 60, and 90 minutes, respectively. Though the concentration of RNA was not affected by delayed freezing, the RNA integrity was decreased with increasing delayed freezing time.

Conclusion

According to the RIN results, we recommend that the collection of colorectal cancer tissue should be done within 10 minutes for studies requiring RNA of high quality and within 30 minutes for usual RNA studies.

Citations

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    Onchira Acharayothin, Benjarat Thiengtrong, Panudeth Juengwiwattanakitti, Panatna Anekwiang, Woramin Riansuwan, Vitoon Chinswangwatanakul, Pariyada Tanjak
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    Separations.2022; 9(11): 324.     CrossRef
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    Biopreservation and Biobanking.2019; 17(4): 282.     CrossRef
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