- Expression of the Cancer Stem Cell Markers CD44 and CD133 in Colorectal Cancer: An Immunohistochemical Staining Analysis
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Injae Hong, Seong Woo Hong, Yeo Goo Chang, Woo Yong Lee, Byungmo Lee, Yun Kyung Kang, You Sun Kim, In Wook Paik, Hyucksang Lee
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Ann Coloproctol. 2015;31(3):84-91. Published online June 30, 2015
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DOI: https://doi.org/10.3393/ac.2015.31.3.84
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The aim of this study was to assess the expressions of CD44 and CD133 in colorectal cancer tissue by using immunohistochemical staining and to analyze the clinical significance of the expressions related to other clinicopathological data and survival results. MethodsOne hundred sixty-two patients with a biopsy-proven colorectal adenocarcinoma who were operated on between January 1998 and August 2004 were enrolled in this study. Immunohistochemical staining for CD44 and CD133 was performed on primary colorectal cancer tissue, metastatic lymph nodes, and synchronous and metachronous metastatic tumor tissues if available. ResultsCD44 expression was stronger in the primary tumor than in metastatic lymph nodes (P < 0.001), and CD133 expression tended to be stronger in primary tumor than in metastatic lymph nodes (P = 0.057). No significant correlation was found between the CD44 and the CD133 expressions. The cases with recurrence showed low expression of CD44 (P = 0.017). CD133 expression was lower in cases with elevated CA 19-9 serum levels (P = 0.028) and advanced T stage (P = 0.038). Multivariate analysis proved that low expression of CD44 was an independent prognosis factor for short disease-free survival (P = 0.028). ConclusionLow CD44 expression was correlated with increased tumor recurrence and short disease-free survival, and low CD133 expression was associated with advanced tumor stage. We suggest that further studies be performed to evaluate whether the immunohistochemical method for determining the CD44 and the CD133 expressions is appropriate for exploring cancer stem-cell biology in patients with colorectal cancer.
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Wei Shi, Xiaoqing Xu, Jinyuan Tian, Ziyi Zhang, Zhanjun Liu New Journal of Chemistry.2024; 48(9): 3793. CrossRef - CD44: A New Prognostic Marker in Colorectal Cancer?
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V. O. Novosad, D. V. Maltseva Bulletin of Experimental Biology and Medicine.2023; 175(1): 144. CrossRef - Immunohistochemical Expression of the Stem Cell Marker CD133 in Colorectal Carcinoma
Sweta Suman, Subhransu Kumar Hota, Pranati Misra, Nageswar Sahu, Subrat Sahu Cureus.2023;[Epub] CrossRef - Cancer stem cells: An overview of the pathophysiological and prognostic roles in colorectal cancer
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Guanglin Cui, Ziqi Wang, Hanzhe Liu, Zhigang Pang Frontiers in Immunology.2022;[Epub] CrossRef - Evaluation and Clinicopathological Correlation of CD44 in Colorectal Adenoma with Low/High-Grade Dysplasia and Carcinoma
Himanshi Bhanu, Ruchi Mittal, Sarojini Raman Clinical Cancer Investigation Journal.2022; 11(6): 9. CrossRef - The correlation of epithelial-mesenchymal transition-related gene expression and the clinicopathologic features of colorectal cancer patients in Taiwan
Yuan-Chang Dai, Chuan-Yin Fang, Hsin-Yi Yang, Yi-Jun Jian, Shou-Chieh Wang, Yi-Wen Liu, Frédéric André PLOS ONE.2021; 16(7): e0254000. CrossRef - Noninvasive Detection of Colorectal Carcinomas Using Serum Protein Biomarkers
Melanie M. Ivancic, Bryant W. Megna, Yuriy Sverchkov, Mark Craven, Mark Reichelderfer, Perry J. Pickhardt, Michael R. Sussman, Gregory D. Kennedy Journal of Surgical Research.2020; 246: 160. CrossRef - Altered expression of fibroblast activation protein-α (FAP) in colorectal adenoma-carcinoma sequence and in lymph node and liver metastases
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Salem Y. Mohamed, Randa Mohamed Kaf, Mona Mostafa Ahmed, Amira Elwan, Hassan R Ashour, Amr Ibrahim Journal of Gastrointestinal Cancer.2019; 50(4): 824. CrossRef - Integrative Analysis of CD133 mRNA in Human Cancers Based on Data Mining
Gui-Min Wen, Fei-Fei Mou, Wei Hou, Dan Wang, Pu Xia Stem Cell Reviews and Reports.2019; 15(1): 23. CrossRef - The Prognostic and Clinical Value of CD44 in Colorectal Cancer: A Meta-Analysis
Zhenpeng Wang, Yufei Tang, Lei Xie, Aiping Huang, Chunchun Xue, Zhen Gu, Kaiqiang Wang, Shaoqi Zong Frontiers in Oncology.2019;[Epub] CrossRef - Modulation of CD44, EGFR and RAC Pathway Genes (WAVE Complex) in Epithelial Cancers
Pranathi Tata, Piyush Gondaliya, Aditya Sunkaria, Akshay Srivastava, Kiran Kalia Current Pharmaceutical Design.2019; 25(8): 833. CrossRef - CD133 expression correlates with clinicopathologic features and poor prognosis of colorectal cancer patients
Rongyong Huang, Dan Mo, Junrong Wu, Huaying Ai, Yiping Lu Medicine.2018; 97(23): e10446. CrossRef - DNA mismatch repair and CD133-marked cancer stem cells in colorectal carcinoma
Phaik-Leng Cheah, Jing Li, Lai-Meng Looi, Kean-Hooi Teoh, Diana Bee-Lan Ong, Mark J. Arends PeerJ.2018; 6: e5530. CrossRef - CD133 and CD44 as cancer stem cell markers in bladder carcinoma
Rania ES. Wasfy, Dina M. El-Guindy Egyptian Journal of Pathology.2017; 37(1): 204. CrossRef - Expression of epithelial-mesenchymal transition and cancer stem cell markers in colorectal adenocarcinoma: Clinicopathological significance
Ji Eun Choi, Jun Sang Bae, Myoung Jae Kang, Myoung Ja Chung, Kyu Yun Jang, Ho Sung Park, Woo Sung Moon Oncology Reports.2017; 38(3): 1695. CrossRef - Targeted photodynamic therapy as potential treatment modality for the eradication of colon cancer and colon cancer stem cells
Natasha Hodgkinson, Cherie A Kruger, Heidi Abrahamse Tumor Biology.2017; 39(10): 101042831773469. CrossRef - Prognostic value of pretreatment serum carbohydrate antigen 19-9 level in patients with colorectal cancer: A meta-analysis
Zhan Yu, Zhen Chen, Jian Wu, Zhong Li, Yugang Wu, Surinder K. Batra PLOS ONE.2017; 12(11): e0188139. CrossRef - Temporal and spatial changes of cells positive for stem-like markers in different compartments and stages of human colorectal adenoma-carcinoma sequence
Guanglin Cui, Gang Xu, Li Zhu, Zhigang Pang, Wei Zheng, Zhenfeng Li, Aping Yuan Oncotarget.2017; 8(28): 45311. CrossRef - A comparison of cancer stem cell markers and nonclassical major histocompatibility complex antigens in colorectal tumor and noncancerous tissues
Rabia Bilge Özgül Özdemir, Alper Tunga Özdemir, Fatih Oltulu, Kamile Kurt, Gürkan Yiğittürk, Cengiz Kırmaz Annals of Diagnostic Pathology.2016; 25: 60. CrossRef - Implications of OCT4 in breast carcinoma from initiation to lymph node metastasis
Hoda H. Abou Gabal, Riham M. Abu-Zeid, Maissa N. El-Maraghy Egyptian Journal of Pathology.2016; 36(2): 194. CrossRef - CD133 expression may be useful as a prognostic indicator in colorectal cancer, a tool for optimizing therapy and supportive evidence for the cancer stem cell hypothesis: a meta-analysis
Yang Zhao, Jing Peng, Enlong Zhang, Ning Jiang, Jiang Li, Qi Zhang, Xuening Zhang, Yuanjie Niu Oncotarget.2016; 7(9): 10023. CrossRef - Prognostic significance of CD44 in human colon cancer and gastric cancer: Evidence from bioinformatic analyses
Pu Xia, Xiao-Yan Xu Oncotarget.2016; 7(29): 45538. CrossRef - Clinical Implication of Colorectal Cancer Stem Cells: Still Has a Long Way to Go
Hungdai Kim Annals of Coloproctology.2015; 31(3): 79. CrossRef - Advances and perspectives of colorectal cancer stem cell vaccine
Mei Guo, Jun Dou Biomedicine & Pharmacotherapy.2015; 76: 107. CrossRef
- Matrix Metalloproteinase-2 and -7 Expression in Colorectal Cancer
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Seong Woo Hong, Yun Kyung Kang, Byungmo Lee, Woo Yong Lee, Yeo Gu Jang, In Wook Paik, Hyucksang Lee
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J Korean Soc Coloproctol. 2011;27(3):133-139. Published online June 30, 2011
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DOI: https://doi.org/10.3393/jksc.2011.27.3.133
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Matrix metalloproteinase-2 (MMP-2) and MMP-7 have been implicated in tumor growth and metastasis. This study aimed to investigate the expressions of MMP-2 and -7 in colorectal cancer and to evaluate their values as prognostic markers. MethodsImmunohistochemical staining for MMP-2 and -7 was done in 144 resected colorectal cancer specimens. Clinicopathological data and survival results were compared with regard to the expression results. ResultsThe expression rates of MMP-2 in tumor cells in the tumor center and the tumor border were 16.7% and 38.9%, respectively. That of MMP-2 in stromal cells was 27.8%. MMP-7 immunoreactivities of tumor cells in the tumor center and the tumor border were 6.9% and 23.6%. The expressions of MMP-2 and MMP-7 were correlated. MMP-2 expression in stromal cells was more increased in the distal part of the colorectum: 8.8% in right colon cancer, 29.5% in left colon cancer and 36.4% in rectal cancer. MMP-2 expression of tumor cells in the tumor border was correlated with T-stage. MMP-7 expression of tumor cells in the tumor border was increased in case of infiltrative cancer compared with fungating tumor. The expression patterns of MMP-2 and -7 were not correlated with other clinicopathological factors, including tumor markers, node metastasis, distant metastasis, lymphatic invasion, tumor differentiation, and recurrence. No significant associations between the overall and disease-free survival rates and the MMP-2 and -7 expression patterns were noted. ConclusionThe high expression rates of MMP-2 and -7 in tumor borders suggest that MMP-2 and -7 have some role in tumor invasion, but in this study, MMP-2 and -7 did not appear to be significant predictors of prognosis in colorectal cancer.
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- A review of matrix metalloproteinase-2-sensitive nanoparticles as a novel drug delivery for tumor therapy
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Otto Luiz Dutra Cerqueira, Mayara Carolline Silva Botelho, Ana Paula Zen Petisco Fiore, Cynthia Aparecida Bueno de Toledo Osório, Rebeka Tomasin, Mauro César Cafundó Morais, Rossana Verónica Mendoza López, Elaine Cristina Cardoso, Santiago Andres Vilella- Neoplasia.2022; 30: 100803. CrossRef - Effect of ginger extracts on colorectal cancer HCT-116 cell line in the expression of MMP-2 and KRAS
Shiva Malmir, Asa Ebrahimi, Frouzandeh Mahjoubi Gene Reports.2020; 21: 100824. CrossRef - Proteome Heterogeneity in Colorectal Cancer
Lay Cheng Lim, Yang Mooi Lim PROTEOMICS.2018;[Epub] CrossRef - Implications of Isoprostanes and Matrix Metalloproteinase-7 Having Potential Role in the Development of Colorectal Cancer in Males
Mahmood Rasool, Arif Malik, Ahmad Ashar Ghuman, Muhammad Abdul Basit Ashraf, Mahwish Arooj, Sulayman Waquar, Sara Zahid, Sumera Shaheen, Aamer Qazi, Muhammad Imran Naseer, Mazin A. Zamzami, Ayat Al-Ghafari, Othman A. Baothman, Mustafa Zeyadi, Nawal Helmi, Frontiers in Oncology.2018;[Epub] CrossRef - Circulating gelatinases are not prognostic of treatment response and survival in locally advanced rectal cancer patients undergoing preoperative chemoradiotherapy
Sukran Ulger, Diclehan Kilic, Fatih Demircioglu, Canan Y Demirtas, Ozge T. Pasaoglu Journal of Cancer Research and Therapeutics.2018; 14(Suppl 1): S90. CrossRef - Profile of Expression of Genes Encoding Matrix Metallopeptidase 9 (MMP9), Matrix Metallopeptidase 28 (MMP28) and TIMP Metallopeptidase Inhibitor 1 (TIMP1) in Colorectal Cancer: Assessment of the Role in Diagnosis and Prognostication
Zbigniew Lorenc, Dariusz Waniczek, Katarzyna Lorenc-Podgórska, Wiktor Krawczyk, Maciej Domagała, Mateusz Majewski, Urszula Mazurek Medical Science Monitor.2017; 23: 1305. CrossRef - Matrix Metalloproteinase-2 and -9, Lactate, and Malate Dehydrogenase and Lipid Peroxides in Sera of Patients with Colorectal Carcinoma
Kristina Gopcevic, B. Rovcanin, D. Kekic, Z. Krivokapic, V. Dragutinovic Folia Biologica.2017; 63(5-6): 190. CrossRef - Prognostic significance of plasma matrix metalloprotease-2 in pancreatic cancer patients
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Chang-long Xu, Jian-zhang Wang, Xuan-ping Xia, Chen-wei Pan, Xiao-xiao Shao, Sheng-long Xia, Shou-xing Yang, Bo Zheng Biochemical and Biophysical Research Communications.2016; 470(2): 397. CrossRef - Shikonin inhibits the cell viability, adhesion, invasion and migration of the human gastric cancer cell line MGC-803 via the Toll-like receptor 2/nuclear factor-kappa B pathway
Ji Ping Liu, Dan Liu, Jun Fei Gu, Mao Mao Zhu, Li Cui Journal of Pharmacy and Pharmacology.2015; 67(8): 1143. CrossRef - Prognostic significance of MMP-7 expression in colorectal cancer: A meta-analysis
Da-wei Sun, Ying-yi Zhang, Yue Qi, Xing-tong Zhou, Guo-yue Lv Cancer Epidemiology.2015; 39(2): 135. CrossRef - Immunoexpression of metalloproteinase 14 and tissue inhibitor of metalloproteinase 2 in colorectal carcinomas and lymph node metastases
Francisco Nélson Nóbrega Furtado, João Paulo Aguiar Sampaio, João Tarcisio Alves Maia-Filho, Renato Braga Vieira, Roberto César Pereira Lima-Júnior, Ronaldo Albuquerque Ribeiro, Paulo Roberto Carvalho Almeida Comparative Clinical Pathology.2015; 24(6): 1367. CrossRef - MMP-2/9-oriented combinations enhance antitumor efficacy of EGFR/HER2-targeting fusion proteins and gemcitabine
YE QIN, XIU-JUN LIU, LIANG LI, XU-JIE LIU, YI LI, RUI-JUAN GAO, RONG-GUANG SHAO, YONG-SU ZHEN Oncology Reports.2014; 32(1): 121. CrossRef - Prognostic Role of Tumor-Associated Proteases in Colorectal Cancer
N. E. Kushlinskii, E. S. Gershtein, E. A. Korotkova, V. V. Prorokov Bulletin of Experimental Biology and Medicine.2013; 154(3): 365. CrossRef - Novel roles of liver sinusoidal endothelial cell lectin in colon carcinoma cell adhesion, migration and in-vivo metastasis to the liver
Yunfei Zuo, Shuangyi Ren, Min Wang, Biao Liu, Juntao Yang, Xuezhang Kuai, Changwei Lin, Dianyuan Zhao, Li Tang, Fuchu He Gut.2013; 62(8): 1169. CrossRef - Patterns of FOXE1 Expression in Papillary Thyroid Carcinoma by Immunohistochemistry
Andrey Bychkov, Vladimir Saenko, Masahiro Nakashima, Norisato Mitsutake, Tatiana Rogounovitch, Alyaksandr Nikitski, Florence Orim, Shunichi Yamashita Thyroid.2013; 23(7): 817. CrossRef - Matrix metalloproteinase 2 overexpression and prognosis in colorectal cancer: a meta-analysis
Mumu Shi, Bo Yu, Hongguo Gao, Jingwen Mu, Changwei Ji Molecular Biology Reports.2013; 40(1): 617. CrossRef
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